User:Michael Strong/H1N1

2009 H1N1 swine flu genome and protein analysis

home • H1N1 •  PB2 • PB1 •  PA  • HA •  NP •  NA •  MP •  NS

Shown below are Protein Structural Homology Models of the PB2, PA, HA, NP, NA, MP1, MP2, NS1 and NS2 proteins from the 2009 Mexico A/Mexico/InDRE4487/2009 H1N1 Swine flu genome. Sequence polymorphisms of the PB2, PB1, PA, HA, NP, NA, MP1, MP2, NS1 and NS2 proteins are also indicated, as identified by comparison of subsequently sequenced 2009 H1N1 strains from around the globe. The "green links" next to the listed amino acids highlight the amino acid polymorphisms in the structural homology models. More detail on each protein, including Multiple Sequence Alignments of newly sequence H1N1 strains, Sequence Polymorphisms, and Related Sequence Homologs are provided on the individual protein pages (links above).

Genome and protein sequences obtained from: http://www.ncbi.nlm.nih.gov/genomes/FLU/SwineFlu.html. Multiple sequence alignments of the PB2, PB1, PA, HA, NP, NA, MP, and NS proteins from 2009 H1N1 strains from Mexico, California, Texas, New York, and Canada are also provided below.

PB2 Protein Structural Homolgy Model


PA Protein Structural Homolgy Model


HA Protein Structural Homolgy Model


HA Sequence Polymorphisms
All Polymorphisms

NP Protein Structural Homolgy Model


NP Sequence Polymorphisms
All Polymorphisms

NA Protein Structural Homolgy Model


NA Sequence Polymorphisms
All Polymorphisms

MP1 Protein Structural Homolgy Model


MP2 Protein Structural Homolgy Model


NS1 Protein Structural Homolgy Model


NS1 Sequence Polymorphisms
All Polymorphisms

NS2 Protein Structural Homolgy Model


NS2 Sequence Polymorphisms
All Polymorphisms

multiple sequence alignment of PB2
CLUSTAL 2.0.10 multiple sequence alignment

PB2 CLUSTAL 2.0.10 = multiple sequence alignment of PB2

A/California/04/2009        MERIKELRDLMSQSRTREILTKTTVDHMAIIKKYTSGRQEKNPALRMKWM 50 A/New_York/18/2009          MERIKELRDLMSQSRTREILTKTTVDHMAIIKKYTSGRQEKNPALRMKWM 50 A/Canada-ON/RV1527/2009     MERIKELRDLMSQSRTREILTKTTVDHMAIIKKYTSGRQEKNPALRMKWM 50 A/Texas/09/2009             MERIKELRDLMSQSRTREILTKTTVDHMAIIKKYTSGRQEKNPALRMKWM 50 A/Mexico/InDRE4487/2009     MERIKELRDLMSQSRTREILTKTTVDHMAIIKKYTSGRQEKNPALRMKWM 50 **************************************************

A/California/04/2009        MAMRYPITADKRIMDMIPERNEQGQTLWSKTNDAGSDRVMVSPLAVTWWN 100 A/New_York/18/2009          MAMRYPITADKRIMDMIPERNEQGQTLWSKTNDAGSDRVMVSPLAVTWWN 100 A/Canada-ON/RV1527/2009     MAMRYPITADKRIMDMIPERNEQGQTLWSKTNDAGSDRVMVSPLAVTWWN 100 A/Texas/09/2009             MAMRYPITADKRIMDMIPERNEQGQTLWSKTNDAGSDRVMVSPLAVTWWN 100 A/Mexico/InDRE4487/2009     MAMRYPITADKRIMDMIPERNEQGQTLWSKTSDAGSDRVMVSPLAVTWWN 100 *******************************.******************

A/California/04/2009        RNGPTTSTVHYPKVYKTYFEKVERLKHGTFGPVHFRNQVKIRRRVDTNPG 150 A/New_York/18/2009          RNGPTTSTVHYPKVYKTYFEKVERLKHGTFGPVHFRNQVKIRRRVDTNPG 150 A/Canada-ON/RV1527/2009     RNGPTTSTVHYPKVYKTYFEKVERLKHGTFGPVHFRNQVKIRRRVDTNPG 150 A/Texas/09/2009             RNGPTTSTVHYPKVYKTYFEKVERLKHGTFGPVHFRNQVKIRRRVDTNPG 150 A/Mexico/InDRE4487/2009     RNGPTTSTVHYPKVYKTYFEKVERLKHGTFGPVHFRNQVKIRRRVDTNPG 150 **************************************************

A/California/04/2009        HADLSAKEAQDVIMEVVFPNEVGARILTSESQLAITKEKKEELQDCKIAP 200 A/New_York/18/2009          HADLSAKEAQDVIMEVVFPNEVGARILTSESQLAITKEKKEELQDCKIAP 200 A/Canada-ON/RV1527/2009     HADLSAKEAQDVIMEVVFPNEVGARILTSESQLAITKEKKEELQDCKIAP 200 A/Texas/09/2009             HADLSAKEAQDVIMEVVFPNEVGARILTSESQLAITKEKKEELQDCKIAP 200 A/Mexico/InDRE4487/2009     HADLSAKEAQDVIMEVVFPNEVGARILTSESQLAITKEKKEELQDCKIAP 200 **************************************************

A/California/04/2009        LMVAYMLERELVRKTRFLPVAGGTGSVYIEVLHLTQGTCWEQMYTPGGEV 250 A/New_York/18/2009          LMVAYMLERELVRKTRFLPVAGGTGSVYIEVLHLTQGTCWEQMYTPGGEV 250 A/Canada-ON/RV1527/2009     LMVAYMLERELVRKTRFLPVAGGTGSVYIEVLHLTQGTCWEQMYTPGGEV 250 A/Texas/09/2009             LMVAYMLERELVRKTRFLPVAGGTGSVYIEVLHLTQGTCWEQMYTPGGEV 250 A/Mexico/InDRE4487/2009     LMVAYMLERELVRKTRFLPVAGGTGSVYIEVLHLTQGTCWEQMYTPGGEV 250 **************************************************

A/California/04/2009        RNDDVDQSLIIAARNIVRRAAVSADPLASLLEMCHSTQIGGVRMVDILRQ 300 A/New_York/18/2009          RNDDVDQSLIIAARNIVRRAAVSADPLASLLEMCHSTQIGGVRMVDILRQ 300 A/Canada-ON/RV1527/2009     RNDDVDQSLIIAARNIVRRAAVSADPLASLLEMCHSTQIGGVRMVDILRQ 300 A/Texas/09/2009             RNDDVDQSLIIAARNIVRRAAVSADPLASLLEMCHSTQIGGVRMVDILRQ 300 A/Mexico/InDRE4487/2009     RNDDVDQSLIIAARNIVRRAAVSADPLASLLEMCHSTQIGGVRMVDILRQ 300 **************************************************

A/California/04/2009        NPTEEQAVDICKAAIGLRISSSFSFGGFTFKRTSGSSVKKEEEVLTGNLQ 350 A/New_York/18/2009          NPTEEQAVDICKAAIGLRISSSFSFGGFTFKRTSGSSVKKEEEVLTGNLQ 350 A/Canada-ON/RV1527/2009     NPTEEQAVDICKAAIGLRISSSFSFGGFTFKRTSGSSVKKEEEVLTGNLQ 350 A/Texas/09/2009             NPTEEQAVDICKAAIGLRISSSFSFGGFTFKRTSGSSVKKEEEVLTGNLQ 350 A/Mexico/InDRE4487/2009     NPTEEQAVDICKAAIGLRISSSFSFGGFTFKRTSGSSVKKEEEVLTGNLQ 350 **************************************************

A/California/04/2009        TLKIRVHEGYEEFTMVGRRATAILRKATRRLIQLIVSGRDEQSIAEAIIV 400 A/New_York/18/2009          TLKIRVHEGYEEFTMVGRRATAILRKATRRLIQLIVSGRDEQSIAEAIIV 400 A/Canada-ON/RV1527/2009     TLKIRVHEGYEEFTMVGRRATAILRKATRRLIQLIVSGRDEQSIAEAIIV 400 A/Texas/09/2009             TLKIRVHEGYEEFTMVGRRATAILRKATRRLIQLIVSGRDEQSIAEAIIV 400 A/Mexico/InDRE4487/2009     TLKIRVHEGYEEFTMVGRRATAILRKATRRLIQLIVSGRDEQSIAEAIIV 400 **************************************************

A/California/04/2009        AMVFSQEDCMIKAVRGDLNFVNRANQRLNPMHQLLRHFQKDAKVLFQNWG 450 A/New_York/18/2009          AMVFSQEDCMIKAVRGDLNFVNRANQRLNPMHQLLRHFQKDAKVLFQNWG 450 A/Canada-ON/RV1527/2009     AMVFSQEDCMIKAVRGDLNFVNRANQRLNPMHQLLRHFQKDAKVLFQNWG 450 A/Texas/09/2009             AMVFSQEDCMIKAVRGDLNFVNRANQRLNPMHQLLRHFQKDAKVLFQNWG 450 A/Mexico/InDRE4487/2009     AMVFSQEDCMIKAVRGDLNFVNRANQRLNPMHQLLRHFQKDAKVLFQNWG 450 **************************************************

A/California/04/2009        IESIDNVMGMIGILPDMTPSTEMSLRGIRVSKMGVDEYSSTERVVVSIDR 500 A/New_York/18/2009          IESIDNVMGMIGILPDMTPSTEMSLRGIRVSKMGVDEYSSTERVVVSIDR 500 A/Canada-ON/RV1527/2009     IESIDNVMGMIGILPDMTPSTEMSLRGIRVSKMGVDEYSSTERVVVSIDR 500 A/Texas/09/2009             IESIDNVMGMIGILPDMTPSTEMSLRGIRVSKMGVDEYSSTERVVVSIDR 500 A/Mexico/InDRE4487/2009     IESIDNVMGMIGILPDMTPSTEMSLRGIRVSKMGVDEYSSTERVVVSIDR 500 **************************************************

A/California/04/2009        FLRVRDQRGNVLLSPEEVSETQGTEKLTITYSSSMMWEINGPESVLVNTY 550 A/New_York/18/2009          FLRVRDQRGNVLLSPEEVSETQGTEKLTITYSSSMMWEINGPESVLVNTY 550 A/Canada-ON/RV1527/2009     FLRVRDQRGNVLLSPEEVSETQGTEKLTITYSSSMMWEINGPESVLVNTY 550 A/Texas/09/2009             FLRVRDQRGNVLLSPEEVSETQGTEKLTITYSSSMMWEINGPESVLVNTY 550 A/Mexico/InDRE4487/2009     FLRVRDQRGNVLLSPEEVSETQGTEKLTITYSSSMMWEINGPESVLVNTY 550 **************************************************

A/California/04/2009        QWIIRNWEIVKIQWSQDPTMLYNKMEFEPFQSLVPKATRSRYSGFVRTLF 600 A/New_York/18/2009          QWIIRNWEIVKIQWSQDPTMLYNKMEFEPFQSLVPKATRSRYSGFVRTLF 600 A/Canada-ON/RV1527/2009     QWIIRNWEIVKIQWSQDPTMLYNKMEFEPFQSLVPKATRSRYSGFVRTLF 600 A/Texas/09/2009             QWIIRNWEIVKIQWSQDPTMLYNKMEFEPFQSLVPKATRSRYSGFVRTLF 600 A/Mexico/InDRE4487/2009     QWIIRNWEIVKIQWSQDPTMLYNKMEFEPFQSLVPKATRSRYSGFVRTLF 600 **************************************************

A/California/04/2009        QQMRDVLGTFDTVQIIKLLPFAAAPPEQSRMQFSSLTVNVRGSGLRILVR 650 A/New_York/18/2009          QQMRDVLGTFDTVQIIKLLPFAAAPPEQSRMQFSSLTVNVRGSGLRILVR 650 A/Canada-ON/RV1527/2009     QQMRDVLGTFDTVQIIKLLPFAAAPPEQSRMQFSSLTVNVRGSGLRILVR 650 A/Texas/09/2009             QQMRDVLGTFDTVQIIKLLPFAAAPPEQSRMQFSSLTVNVRGSGLRILVR 650 A/Mexico/InDRE4487/2009     QQMRDVLGTFDTVQIIKLLPFAAAPPEQSRMQFSSLTVNVRGSGLRILVR 650 **************************************************

A/California/04/2009        GNSPVFNYNKATKRLTVLGKDAGALTEDPDEGTSGVESAVLRGFLILGKE 700 A/New_York/18/2009          GNSPVFNYNKATKRLTVLGKDAGALTEDPDEGTSGVESAVLRGFLILGKE 700 A/Canada-ON/RV1527/2009     GNSPVFNYNKATKRLTVLGKDAGALTEDPDEGTSGVESAVLRGFLILGKE 700 A/Texas/09/2009             GNSPVFNYNKATKRLTVLGKDAGALTEDPDEGTSGVESAVLRGFLILGKE 700 A/Mexico/InDRE4487/2009     GNSPVFNYNKATKRLTVLGKDAGALTEDPDEGTSGVESAVLRGFLILGKE 700 **************************************************

A/California/04/2009        DKRYGPALSINELSNLAKGEKANVLIGQGDVVLVMKRKRDSSILTDSQTA 750 A/New_York/18/2009          DKRYGPALSINELSNLAKGEKANVLIGQGDVVLVMKRKRDSSILTDSQTA 750 A/Canada-ON/RV1527/2009     DKRYGPALSINELSNLAKGEKANVLIGQGDVVLVMKRKRDSSILTDSQTA 750 A/Texas/09/2009             DKRYGPALSINELSNLAKGEKANVLIGQGDVVLVMKRKRDSSILTDSQTA 750 A/Mexico/InDRE4487/2009     DKRYGPALSINELSNLAKGEKANVLIGQGDVVLVMKRKRDSSILTDSQTA 750 **************************************************

A/California/04/2009        TKRIRMAIN 759 A/New_York/18/2009          TKRIRMAIN 759 A/Canada-ON/RV1527/2009     TKRIRMAIN 759 A/Texas/09/2009             TKRIRMAIN 759 A/Mexico/InDRE4487/2009     TKRIRMAIN 759 ********* <SPAN class=3Dpsmall>

multiple sequence alignment of PB1
PB1 CLUSTAL 2.0.10 = multiple sequence alignment of PB1

A/Canada-ON/RV1527/2009     MDVNPTLLFLKIPAQNAISTTFPYTGDPPYSHGTGTGYTMDTVNRTHQYS 50 A/Texas/09/2009             MDVNPTLLFLKIPAQNAISTTFPYTGDPPYSHGTGTGYTMDTVNRTHQYS 50 A/Mexico/InDRE4487/2009     MDVNPTLLFLKIPAQNAISTTFPYTGDPPYSHGTGTGYTMDTVNRTHQYS 50 A/California/04/2009        MDVNPTLLFLKIPAQNAISTTFPYTGDPPYSHGTGTGYTMDTVNRTHQYS 50 A/New_York/18/2009          MDVNPTLLFLKIPAQNAISTTFPYTGDPPYSHGTGTGYTMDTVNRTHQYS 50 **************************************************

A/Canada-ON/RV1527/2009     EKGKWTTNTETGAPQLNPIDGPLPEDNEPSGYAQTDCVLEAMAFLEESHP 100 A/Texas/09/2009             EKGKWTTNTETGAPQLNPIDGPLPEDNEPSGYAQTDCVLEAMAFLEESHP 100 A/Mexico/InDRE4487/2009     EKGKWTTNTETGAPQLNPIDGPLPEDNEPSGYAQTDCVLEAMAFLEESHP 100 A/California/04/2009        EKGKWTTNTETGAPQLNPIDGPLPEDNEPSGYAQTDCVLEAMAFLEESHP 100 A/New_York/18/2009          EKGKWTTNTETGAPQLNPIDGPLPEDNEPSGYAQTDCVLEAMAFLEESHP 100 **************************************************

A/Canada-ON/RV1527/2009     GIFENSCLETMEVVQQTRVDKLTQGRQTYDWTLNRNQPAATALANTIEVF 150 A/Texas/09/2009             GIFENSCLETMEVVQQTRVDKLTQGRQTYDWTLNRNQPAATALANTIEVF 150 A/Mexico/InDRE4487/2009     GIFENSCLETMEVVQQTRVDKLTQGRQTYDWTLNRNQPAATALANTIEVF 150 A/California/04/2009        GIFENSCLETMEVVQQTRVDKLTQGRQTYDWTLNRNQPAATALANTIEVF 150 A/New_York/18/2009          GIFENSCLETMEVVQQTRVDKLTQGRQTYDWTLNRNQPAATALANTIEVF 150 **************************************************

A/Canada-ON/RV1527/2009     RSNGLTANESGRLIDFLKDVMESMNKEEIEITTHFQRKRRVRDNMTKKMV 200 A/Texas/09/2009             RSNGLTANESGRLIDFLKDVMESMNKEEIEITTHFQRKRRVRDNMTKKMV 200 A/Mexico/InDRE4487/2009     RSNGLTANESGRLIDFLKDVMESMNKEEIEITTHFQRKRRVRDNMTKKMV 200 A/California/04/2009        RSNGLTANESGRLIDFLKDVMESMNKEEIEITTHFQRKRRVRDNMTKKMV 200 A/New_York/18/2009          RSNGLTANESGRLIDFLKDVMESMNKEEIEITTHFQRKRRVRDNMTKKMV 200 **************************************************

A/Canada-ON/RV1527/2009     TQRTIGKKKQRLNKRGYLIRALTLNTMTKDAERGKLKRRAIATPGMQIRG 250 A/Texas/09/2009             TQRTIGKKKQRLNKRGYLIRALTLNTMTKDAERGKLKRRAIATPGMQIRG 250 A/Mexico/InDRE4487/2009     TQRTIGKKKQRLNKRGYLIRALTLNTMTKDAERGKLKRRAIATPGMQIRG 250 A/California/04/2009        TQRTIGKKKQRLNKRGYLIRALTLNTMTKDAERGKLKRRAIATPGMQIRG 250 A/New_York/18/2009          TQRTIGKKKQRLNKRGYLIRALTLNTMTKDAERGKLKRRAIATPGMQIRG 250 **************************************************

A/Canada-ON/RV1527/2009     FVYFVETLARSICEKLEQSGLPVGGNEKKAKLANVVRKMMTNSQDTEISF 300 A/Texas/09/2009             FVYFVETLARSICEKLEQSGLPVGGNEKKAKLANVVRKMMTNSQDTEISF 300 A/Mexico/InDRE4487/2009     FVYFVETLARSICEKLEQSGLPVGGNEKKAKLANVVRKMMTNSQDTEISF 300 A/California/04/2009        FVYFVETLARSICEKLEQSGLPVGGNEKKAKLANVVRKMMTNSQDTEISF 300 A/New_York/18/2009          FVYFVETLARSICEKLEQSGLPVGGNEKKAKLANVVRKMMTNSQDTEISF 300 **************************************************

A/Canada-ON/RV1527/2009     TITGDNTKWNENQNPRMFLAMITYITRNQPEWFRNILSMAPIMFSNKMAR 350 A/Texas/09/2009             TITGDNTKWNENQNPRMFLAMITYITRNQPEWFRNILSMAPIMFSNKMAR 350 A/Mexico/InDRE4487/2009     TITGDNTKWNENQNPRMFLAMITYITRNQPEWFRNILSMAPIMFSNKMAR 350 A/California/04/2009        TITGDNTKWNENQNPRMFLAMITYITRNQPEWFRNILSMAPIMFSNKMAR 350 A/New_York/18/2009          TITGDNTKWNENQNPRMFLAMITYITRNQPEWFRNILSMAPIMFSNKMAR 350 **************************************************

A/Canada-ON/RV1527/2009     LGKGYMFESKRMKIRTQIPAEMLASIDLKYFNESTKKKIEKIRPLLIDGT 400 A/Texas/09/2009             LGKGYMFESKRMKIRTQIPAEMLASIDLKYFNESTKKKIEKIRPLLIDGT 400 A/Mexico/InDRE4487/2009     LGKGYMFESKRMKIRTQIPAEMLASIDLKYFNESTKKKIEKIRPLLIDGT 400 A/California/04/2009        LGKGYMFESKRMKIRTQIPAEMLASIDLKYFNESTKKKIEKIRPLLIDGT 400 A/New_York/18/2009          LGKGYMFESKRMKIRTQIPAEMLASIDLKYFNESTKKKIEKIRPLLIDGT 400 **************************************************

A/Canada-ON/RV1527/2009     ASLSPGMMMGMFNMLSTVLGVSILNLGQKKYTKTIYWWDGLQSSDDFALI 450 A/Texas/09/2009             ASLSPGMMMGMFNMLSTVLGVSILNLGQKKYTKTIYWWDGLQSSDDFALI 450 A/Mexico/InDRE4487/2009     ASLSPGMMMGMFNMLSTVLGVSILNLGQKKYTKTIYWWDGLQSSDDFALI 450 A/California/04/2009        ASLSPGMMMGMFNMLSTVLGVSILNLGQKKYTKTIYWWDGLQSSDDFALI 450 A/New_York/18/2009          ASLSPGMMMGMFNMLSTVLGVSILNLGQKKYTKTIYWWDGLQSSDDFALI 450 **************************************************

A/Canada-ON/RV1527/2009     VNAPNHEGIQAGVDRFYRTCKLVGINMSKKKSYINKTGTFEFTSFFYRYG 500 A/Texas/09/2009             VNAPNHEGIQAGVDRFYRTCKLVGINMSKKKSYINKTGTFEFTSFFYRYG 500 A/Mexico/InDRE4487/2009     VNAPNHEGIQAGVDRFYRTCKLVGINMSKKKSYINKTGTFEFTSFFYRYG 500 A/California/04/2009        VNAPNHEGIQAGVDRFYRTCKLVGINMSKKKSYINKTGTFEFTSFFYRYG 500 A/New_York/18/2009          VNAPNHEGIQAGVDRFYRTCKLVGINMSKKKSYINKTGTFEFTSFFYRYG 500 **************************************************

A/Canada-ON/RV1527/2009     FVANFSMELPSFGVSGVNESADMSIGVTVIKNNMINNDLGPATAQMALQL 550 A/Texas/09/2009             FVANFSMELPSFGVSGVNESADMSIGVTVIKNNMINNDLGPATAQMALQL 550 A/Mexico/InDRE4487/2009     FVANFSMELPSFGVSGVNESADMSIGVTVIKNNMINNDLGPATAQMALQL 550 A/California/04/2009        FVANFSMELPSFGVSGVNESADMSIGVTVIKNNMINNDLGPATAQMALQL 550 A/New_York/18/2009          FVANFSMELPSFGVSGVNESADMSIGVTVIKNNMINNDLGPATAQMALQL 550 **************************************************

A/Canada-ON/RV1527/2009     FIKDYRYTYRCHRGDTQIQTRRSFELKKLWDQTQSKVGLLVSDGGPNLYN 600 A/Texas/09/2009             FIKDYRYTYRCHRGDTQIQTRRSFELKKLWDQTQSKVGLLVSDGGPNLYN 600 A/Mexico/InDRE4487/2009     FIKDYRYTYRCHRGDTQIQTRRSFELKKLWDQTQSKVGLLVSDGGPNLYN 600 A/California/04/2009        FIKDYRYTYRCHRGDTQIQTRRSFELKKLWDQTQSKVGLLVSDGGPNLYN 600 A/New_York/18/2009          FIKDYRYTYRCHRGDTQIQTRRSFELKKLWDQTQSKVGLLVSDGGPNLYN 600 **************************************************

A/Canada-ON/RV1527/2009     IRNLHIPEVCLKWELMDDDYRGRLCNPLNPFVSHKEIDSVNNAVVMPAHG 650 A/Texas/09/2009             IRNLHIPEVCLKWELMDDDYRGRLCNPLNPFVSHKEIDSVNNAVVMPAHG 650 A/Mexico/InDRE4487/2009     IRNLHIPEVCLKWELMDDDYRGRLCNPLNPFVSHKEIDSVNNAVVMPAHG 650 A/California/04/2009        IRNLHIPEVCLKWELMDDDYRGRLCNPLNPFVSHKEIDSVNNAVVMPAHG 650 A/New_York/18/2009          IRNLHIPEVCLKWELMDDDYRGRLCNPLNPFVSHKEIDSVNNAVVMPAHG 650 **************************************************

A/Canada-ON/RV1527/2009     PAKSMEYDAVATTHSWIPKRNRSILNTSQRGILEDEQMYQKCCNLFEKFF 700 A/Texas/09/2009             PAKSMEYDAVATTHSWIPKRNRSILNTSQRGILEDEQMYQKCCNLFEKFF 700 A/Mexico/InDRE4487/2009     PAKSMEYDAVATTHSWIPKRNRSILNTSQRGILEDEQMYQKCCNLFEKFF 700 A/California/04/2009        PAKSMEYDAVATTHSWIPKRNRSILNTSQRGILEDEQMYQKCCNLFEKFF 700 A/New_York/18/2009          PAKSMEYDAVATTHSWIPKRNRSILNTNQRGILEDEQMYQKCCNLFEKFF 700 ***************************.**********************

A/Canada-ON/RV1527/2009     PSSSYRRPVGISSMVEAMVSRARIDARVDFESGRIKKEEFSEIMKICSTI 750 A/Texas/09/2009             PSSSYRRPVGISSMVEAMVSRARIDARVDFESGRIKKEEFSEIMKICSTI 750 A/Mexico/InDRE4487/2009     PSSSYRRPVGISSMVEAMVSRARIDARVDFESGRIKKEEFSEIMKICSTI 750 A/California/04/2009        PSSSYRRPVGISSMVEAMVSRARIDARVDFESGRIKKEEFSEIMKICSTI 750 A/New_York/18/2009          PSSSYRRPVGISSMVEAMVSRARIDARVDFESGRIKKEEFSEIMKICSTI 750 **************************************************

A/Canada-ON/RV1527/2009     EELRRQK 757 A/Texas/09/2009             EELRRQK 757 A/Mexico/InDRE4487/2009     EELRRQK 757 A/California/04/2009        EELRRQK 757 A/New_York/18/2009          EELRRQK 757 ******* </PRE><SPAN class=3Dpsmall>

multiple sequence alignment of PA
PA CLUSTAL 2.0.10 = multiple sequence alignment of PA

A/Canada-ON/RV1527/2009     MEDFVRQCFNPMIVELAEKAMKEYGEDPKIETNKFAAICTHLEVCFMYSD 50 A/Texas/09/2009             MEDFVRQCFNPMIVELAEKAMKEYGEDPKIETNKFAAICTHLEVCFMYSD 50 A/Mexico/InDRE4487/2009     MEDFVRQCFNPMIVELAEKAMKEYGEDPKIETNKFAAICTHLEVCFMYSD 50 A/New_York/18/2009          MEDFVRQCFNPMIVELAEKAMKEYGEDPKIETNKFAAICTHLEVCFMYSD 50 A/California/04/2009        MEDFVRQCFNPMIVELAEKAMKEYGEDPKIETNKFAAICTHLEVCFMYSD 50 **************************************************

A/Canada-ON/RV1527/2009     FHFIDERGESIIVESGDPNALLKHRFEIIEGRDRIMAWTVVNSICNTTGV 100 A/Texas/09/2009             FHFIDERGESIIVESGDPNALLKHRFEIIEGRDRIMAWTVVNSICNTTGV 100 A/Mexico/InDRE4487/2009     FHFIDERGESIIVESGDPNALLKHRFEIIEGRDRIMAWTVVNSICNTTGV 100 A/New_York/18/2009          FHFIDERGESIIVESGDPNALLKHRFEIIEGRDRIMAWTVVNSICNTTGV 100 A/California/04/2009        FHFIDERGESIIVESGDPNALLKHRFEIIEGRDRIMAWTVVNSICNTTGV 100 **************************************************

A/Canada-ON/RV1527/2009     EKPKFLPDLYDYKENRFIEIGVTRREVHIYYLEKANKIKSEKTHIHIFSF 150 A/Texas/09/2009             EKPKFLPDLYDYKENRFIEIGVTRREVHIYYLEKANKIKSEKTHIHIFSF 150 A/Mexico/InDRE4487/2009     EKPKFLPDLYDYKENRFIEIGVTRREVHIYYLEKANKIKSEKTHIHIFSF 150 A/New_York/18/2009          EKPKFLPDLYDYKENRFIEIGVTRREVHIYYLEKANKIKSEKTHIHIFSF 150 A/California/04/2009        EKPKFLPDLYDYKENRFIEIGVTRREVHIYYLEKANKIKSEKTHIHIFSF 150 **************************************************

A/Canada-ON/RV1527/2009     TGEEMATKADYTLDEESRARIKTRLFTIRQEMASRSLWDSFRQSERGEET 200 A/Texas/09/2009             TGEEMATKADYTLDEESRARIKTRLFTIRQEMASRSLWDSFRQSERGEET 200 A/Mexico/InDRE4487/2009     TGEEMATKADYTLDEESRARIKTRLFTIRQEMASRSLWDSFRQSERGEET 200 A/New_York/18/2009          TGEEMATKADYTLDEESRARIKTRLFTIRQEMASRSLWDSFRQSERGEET 200 A/California/04/2009        TGEEMATKADYTLDEESRARIKTRLFTIRQEMASRSLWDSFRQSERGEET 200 **************************************************

A/Canada-ON/RV1527/2009     IEEKFEITGTMRKLADQSLPPNFSSLENFRAYVDGFEPNGCIEGKLSQMS 250 A/Texas/09/2009             IEEKFEITGTMRKLADQSLPPNFSSLENFRAYVDGFEPNGCIEGKLSQMS 250 A/Mexico/InDRE4487/2009     IEEKFEITGTMRKLADQSLPPNFSSLENFRAYVDGFEPNGCIEGKLSQMS 250 A/New_York/18/2009          IEEKFEITGTMRKLADQSLPPNFSSLENFRAYVDGFEPNGCIEGKLSQMS 250 A/California/04/2009        IEEKFEITGTMRKLADQSLPPNFPSLENFRAYVDGFEPNGCIEGKLSQMS 250 ***********************.**************************

A/Canada-ON/RV1527/2009     KEVNAKIEPFLRTTPRPLRLPDGPLCHQRSKFLLMDALKLSIEDPSHEGE 300 A/Texas/09/2009             KEVNAKIEPFLRTTPRPLRLPDGPLCHQRSKFLLMDALKLSIEDPSHEGE 300 A/Mexico/InDRE4487/2009     KEVNAKIEPFLRTTPRPLRLPDGPICHQRSKFLLMDALKLSIEDPSHEGE 300 A/New_York/18/2009          KEVNAKIEPFLRTTPRPLRLPDGPLCHQRSKFLLMDALKLSIEDPSHEGE 300 A/California/04/2009        KEVNAKIEPFLRTTPRPLRLPDGPLCHQRSKFLLMDALKLSIEDPSHEGE 300 ************************:*************************

A/Canada-ON/RV1527/2009     GIPLYDAIKCMKTFFGWKEPNIVKPHEKGINPNYLMAWKQVLAELQDIEN 350 A/Texas/09/2009             GIPLYDAIKCMKTFFGWKEPNIVKPHEKGINPNYLMAWKQVLAELQDIEN 350 A/Mexico/InDRE4487/2009     GIPLYDAIKCMKTFFGWKEPNIVKPHEKGINPNYLMAWKQVLAELQDIEN 350 A/New_York/18/2009          GIPLYDAIKCMKTFFGWKEPNIVKPHEKGINPNYLMAWKQVLAELQDIEN 350 A/California/04/2009        GIPLYDAIKCMKTFFGWKEPNIVKPHEKGINPNYLMAWKQVLAELQDIEN 350 **************************************************

A/Canada-ON/RV1527/2009     EEKIPRTKNMKRTSQLKWALGENMAPEKVDFDDCKDVGDLKQYDSDEPEP 400 A/Texas/09/2009             EEKIPRTKNMKRTSQLKWALGENMAPEKVDFDDCKDVGDLKQYDSDEPEP 400 A/Mexico/InDRE4487/2009     EEKIPRTKNMKRTSQLKWALGENMAPEKVDFDDCKDVGDLKQYDSDEPEP 400 A/New_York/18/2009          EEKIPRTKNMKRTSQLKWALGENMAPEKVDFDDCKDVGDLKQYDSDEPEP 400 A/California/04/2009        EEKIPRTKNMKRTSQLKWALGENMAPEKVDFDDCKDVGDLKQYDSDEPEP 400 **************************************************

A/Canada-ON/RV1527/2009     RSLASWVQNEFNKACELTDSSWIELDEIGEDVAPIEHIASMRRNYFTAEV 450 A/Texas/09/2009             RSLASWVQNEFNKACELTDSSWIELDEIGEDVAPIEHIASMRRNYFTAEV 450 A/Mexico/InDRE4487/2009     RSLASWVQNEFNKACELTDSSWIELDEIGEDVAPIEHIASMRRNYFTAEV 450 A/New_York/18/2009          RSLASWVQNEFNKACELTDSSWIELDEIGEDVAPIEHIASMRRNYFTAEV 450 A/California/04/2009        RSLASWVQNEFNKACELTDSSWIELDEIGEDVAPIEHIASMRRNYFTAEV 450 **************************************************

A/Canada-ON/RV1527/2009     SHCRATEYIMKGVYINTALLNASCAAMDDFQLIPMISKCRTKEGRRKTNL 500 A/Texas/09/2009             SHCRATEYIMKGVYINTALLNASCAAMDDFQLIPMISKCRTKEGRRKTNL 500 A/Mexico/InDRE4487/2009     SHCRATEYIMKGVYINTALLNASCAAMDDFQLIPMISKCRTKEGRRKTNL 500 A/New_York/18/2009          SHCRATEYIMKGVYINTALLNASCAAMDDFQLIPMISKCRTKEGRRKTNL 500 A/California/04/2009        SHCRATEYIMKGVYINTALLNASCAAMDDFQLIPMISKCRTKEGRRKTNL 500 **************************************************

A/Canada-ON/RV1527/2009     YGFIIKGRSHLRNDTDVVNFVSMEFSLTDPRLEPHKWEKYCVLEIGDMLL 550 A/Texas/09/2009             YGFIIKGRSHLRNDTDVVNFVSMEFSLTDPRLEPHKWEKYCVLEIGDMLL 550 A/Mexico/InDRE4487/2009     YGFIIKGRSHLRNDTDVVNFVSMEFSLTDPRLEPHKWEKYCVLEIGDMLL 550 A/New_York/18/2009          YGFIIKGRSHLRNDTDVVNFVSMEFSLTDPRLEPHKWEKYCVLEIGDMLL 550 A/California/04/2009        YGFIIKGRSHLRNDTDVVNFVSMEFSLTDPRLEPHKWEKYCVLEIGDMLL 550 **************************************************

A/Canada-ON/RV1527/2009     RTAIGQVSRPMFLYVRTNGTSKIKMKWGMEMRRCLLQSLQQIESMIEAES 600 A/Texas/09/2009             RTAIGQVSRPMFLYVRTNGTSKIKMKWGMEMRRCLLQSLQQIESMIEAES 600 A/Mexico/InDRE4487/2009     RTAIGQVSRPMFLYVRTNGTSKIKMKWGMEMRRCLLQSLQQIESMIEAES 600 A/New_York/18/2009          RTAIGQVSRPMFLYVRTNGTSKIKMKWGMEMRRCLLQSLQQIESMIEAES 600 A/California/04/2009        RTAIGQVSRPMFLYVRTNGTSKIKMKWGMEMRRCLLQSLQQIESMIEAES 600 **************************************************

A/Canada-ON/RV1527/2009     SVKEKDMTKEFFENKSETWPIGESPRGVEEGSIGKVCRTLLAKSVFNSLY 650 A/Texas/09/2009             SVKEKDMTKEFFENKSETWPIGESPRGVEEGSIGKVCRTLLAKSVFNSLY 650 A/Mexico/InDRE4487/2009     SVKEKDMTKEFFENKSETWPIGESPRGVEEGSIGKVCRTLLAKSVFNSLY 650 A/New_York/18/2009          SVKEKDMTKEFFENKSETWPIGESPRGVEEGSIGKVCRTLLAKSVFNSLY 650 A/California/04/2009        SVKEKDMTKEFFENKSETWPIGESPRGVEEGSIGKVCRTLLAKSVFNSLY 650 **************************************************

A/Canada-ON/RV1527/2009     ASPQLEGFSAESRKLLLIVQALRDNLEPGTFDLGGLYEAIEECLINDPWV 700 A/Texas/09/2009             ASPQLEGFSAESRKLLLIVQALRDNLEPGTFDLGGLYEAIEECLINDPWV 700 A/Mexico/InDRE4487/2009     ASPQLEGFSAESRKLLLIVQALRDNLEPGTFDLGGLYEAIEECLINDPWV 700 A/New_York/18/2009          ASPQLEGFSAESRKLLLIVQALRDNLEPGTFDLGGLYEAIEECLINDPWV 700 A/California/04/2009        ASPQLEGFSAESRKLLLIVQALRDNLEPGTFDLGGLYEAIEECLINDPWV 700 **************************************************

A/Canada-ON/RV1527/2009     LLNASWFNSFLTHALK 716 A/Texas/09/2009             LLNASWFNSFLTHALK 716 A/Mexico/InDRE4487/2009     LLNASWFNSFLTHALK 716 A/New_York/18/2009          LLNASWFNSFLTHALK 716 A/California/04/2009        LLNASWFNSFLTHALK 716 ****************

</PRE><SPAN class=3Dpsmall>

multiple sequence alignment of HA
HA CLUSTAL 2.0.10 = multiple sequence alignment of HA

A/Texas/04/2009             MKAILVVLLYTFATANADTLCIGYHANNSTDTVDTVLEKNVTVTHSVNLL 50 A/Mexico/InDRE4487/2009     MKAILVVLLYTFATANADTLCIGYHANNSTDTVDTVLEKNVTVTHSVNLL 50 A/New_York/18/2009          MKAILVVLLYTFATANADTLCIGYHANNSTDTVDTVLEKNVTVTHSVNLL 50 A/Canada-ON/RV1527/2009     MKAILVVLLYTFATANADTLCIGYHANNSTDTVDTVLEKNVTVTHSVNLL 50 A/California/04/2009        MKAILVVLLYTFATANADTLCIGYHANNSTDTVDTVLEKNVTVTHSVNLL 50 **************************************************

A/Texas/04/2009             EDKHNGKLCKLRGVAPLHLGKCNIAGWILGNPECESLSTASSWSYIVETS 100 A/Mexico/InDRE4487/2009     EDKHNGKLCKLRGVAPLHLGKCNIAGWILGNPECESLSTASSWSYIVETS 100 A/New_York/18/2009          EDKHNGKLCKLRGVAPLHLGKCNIAGWILGNPECESLSTASSWSYIVETS 100 A/Canada-ON/RV1527/2009     EDKHNGKLCKLRGVAPLHLGKCNIAGWILGNPECESLSTASSWSYIVETS 100 A/California/04/2009        EDKHNGKLCKLRGVAPLHLGKCNIAGWILGNPECESLSTASSWSYIVETP 100 *************************************************.

A/Texas/04/2009             SSDNGTCYPGDFIDYEELREQLSSVSSFERFEIFPKTSSWPNHDSNKGVT 150 A/Mexico/InDRE4487/2009     SSDNGTCYPGDFIDYEELREQLSSVSSFERFEIFPKTSSWPNHDSNKGVT 150 A/New_York/18/2009          SSDNGTCYPGDFIDYEELREQLSSVSSFERFEIFPKTSSWPNHDSNKGVT 150 A/Canada-ON/RV1527/2009     SSDNGTCYPGDFIDYEELREQLSSVSSFERFEIFPKTSSWPNHDSNKGVT 150 A/California/04/2009        SSDNGTCYPGDFIDYEELREQLSSVSSFERFEIFPKTSSWPNHDSNKGVT 150 **************************************************

A/Texas/04/2009             AACPHAGAKSFYKNLIWLVKKGNSYPKLSKSYINDKGKEVLVLWGIHHPS 200 A/Mexico/InDRE4487/2009     AACPHAGAKSFYKNLIWLVKKGNSYPKLSKSYINDKGKEVLVLWGIHHPS 200 A/New_York/18/2009          AACPHAGAKSFYKNLIWLVKKGNSYPKLSKSYINDKGKEVLVLWGIHHPS 200 A/Canada-ON/RV1527/2009     AACPHAGAKSFYKNLIWLVKKGNSYPKLSKSYINDKGKEVLVLWGIHHPS 200 A/California/04/2009        AACPHAGAKSFYKNLIWLVKKGNSYPKLSKSYINDKGKEVLVLWGIHHPS 200 **************************************************

A/Texas/04/2009             TSADQQSLYQNADAYVFVGSSRYSKKFKPEIAIRPKVRDQEGRMNYYWTL 250 A/Mexico/InDRE4487/2009     TSADQQSLYQNADAYVFVGSSRYSKKFKPEIAIRPKVRDQEGRMNYYWTL 250 A/New_York/18/2009          TSADQQSLYQNADAYVFVGTSRYSKKFKPEIAIRPKVRDQEGRMNYYWTL 250 A/Canada-ON/RV1527/2009     TSADQQSLYQNADAYVFVGTSRYSKKFKPEIAIRPKVRDQEGRMNYYWTL 250 A/California/04/2009        TSADQQSLYQNADTYVFVGSSRYSKKFKPEIAIRPKVRDQEGRMNYYWTL 250 *************:*****:******************************

A/Texas/04/2009             VEPGDKITFEATGNLVVPRYAFAMERNAGSGIIISDTPVHDCNTTCQTPK 300 A/Mexico/InDRE4487/2009     VEPGDKITFEATGNLVVPRYAFAMERNAGSGIIISDTPVHDCNTTCQTPK 300 A/New_York/18/2009          VEPGDKITFEATGNLVVPRYAFAMERNAGSGIIISDTPVHDCNTTCQTPK 300 A/Canada-ON/RV1527/2009     VEPGDKITFEATGNLVVPRYAFAMERNAGSGIIISDTPVHDCNTTCQTPK 300 A/California/04/2009        VEPGDKITFEATGNLVVPRYAFAMERNAGSGIIISDTPVHDCNTTCQTPK 300 **************************************************

A/Texas/04/2009             GAINTSLPFQNIHPITIGKCPKYVKSTKLRLATGLRNVPSIQSRGLFGAI 350 A/Mexico/InDRE4487/2009     GAINTSLPFQNIHPITIGKCPKYVKSTKLRLATGLRNVPSIQSRGLFGAI 350 A/New_York/18/2009          GAINTSLPFQNIHPITIGKCPKYVKSTKLRLATGLRNVPSIQSRGLFGAI 350 A/Canada-ON/RV1527/2009     GAINTSLPFQNIHPITIGKCPKYVKSTKLRLATGLRNVPSIQSRGLFGAI 350 A/California/04/2009        GAINTSLPFQNIHPITIGKCPKYVKSTKLRLATGLRNIPSIQSRGLFGAI 350 *************************************:************

A/Texas/04/2009             AGFIEGGWTGMVDGWYGYHHQNEQGSGYAADLKSTQNAIDEITNKVNSVI 400 A/Mexico/InDRE4487/2009     AGFIEGGWTGMVDGWYGYHHQNEQGSGYAADLKSTQNAIDEITNKVNSVI 400 A/New_York/18/2009          AGFIEGGWTGMVDGWYGYHHQNEQGSGYAADLKSTQNAIDEITNKVNSVI 400 A/Canada-ON/RV1527/2009     AGFIEGGWTGMVDGWYGYHHQNEQGSGYAADLKSTQNAIDEITNKVNSVI 400 A/California/04/2009        AGFIEGGWTGMVDGWYGYHHQNEQGSGYAADLKSTQNAIDEITNKVNSVI 400 **************************************************

A/Texas/04/2009             EKMNTQFTAVGKEFNHLEKRIENLNKKVDDGFLDIWTYNAELLVLLENER 450 A/Mexico/InDRE4487/2009     EKMNTQFTAVGKEFNHLEKRIENLNKKVDDGFLDIWTYNAELLVLLENER 450 A/New_York/18/2009          EKMNTQFTAVGKEFNHLEKRIENLNKKVDDGFLDIWTYNAELLVLLENER 450 A/Canada-ON/RV1527/2009     EKMNTQFTAVGKEFNHLEKRIENLNKKVDDGFLDIWTYNAELLVLLENER 450 A/California/04/2009        EKMNTQFTAVGKEFNHLEKRIENLNKKVDDGFLDIWTYNAELLVLLENER 450 **************************************************

A/Texas/04/2009             TLDYHDSNVKNLYEKVRSQLKNNAKEIGNGCFEFYHKCDNTCMESVKNGT 500 A/Mexico/InDRE4487/2009     TLDYHDSNVKNLYEKVRSQLKNNAKEIGNGCFEFYHKCDNTCMESVKNGT 500 A/New_York/18/2009          TLDYHDSNVKNLYEKVRSQLKNNAKEIGNGCFEFYHKCDNTCMESVKNGT 500 A/Canada-ON/RV1527/2009     TLDYHDSNVKNLYEKVRSQLKNNAKEIGNGCFEFYHKCDNTCMESVKNGT 500 A/California/04/2009        TLDYHDSNVKNLYEKVRSQLKNNAKEIGNGCFEFYHKCDNTCMESVKNGT 500 **************************************************

A/Texas/04/2009             YDYPKYSEEAKLNREEIDGVKLESTRIYQILAIYSTVASSLVLVVSLGAI 550 A/Mexico/InDRE4487/2009     YDYPKYSEEAKLNREEIDGVKLESTRIYQILAIYSTVASSLVLVVSLGAI 550 A/New_York/18/2009          YDYPKYSEEAKLNREEIDGVKLESTRIYQILAIYSTVASSLVLVVSLGAI 550 A/Canada-ON/RV1527/2009     YDYPKYSEEAKLNREEIDGVKLESTRIYQILAIYSTVASSLVLVVSLGAI 550 A/California/04/2009        YDYPKYSEEAKLNREEIDGVKLESTRIYQILAIYSTVASSLVLVVSLGAI 550 **************************************************

A/Texas/04/2009             SFWMCSNGSLQCRICI 566 A/Mexico/InDRE4487/2009     SFWMCSNGSLQCRICI 566 A/New_York/18/2009          SFWMCSNGSLQCRICI 566 A/Canada-ON/RV1527/2009     SFWMCSNGSLQCRICI 566 A/California/04/2009        SFWMCSNGSLQCRICI 566 **************** </PRE><SPAN class=3Dpsmall>

multiple sequence alignment of NP
NP CLUSTAL 2.0.10 = multiple sequence alignment of NP

A/California/04/2009        --MASQGTKRSYEQMETGGERQDATEIRASVGRMIGGIGRFYIQMC 44 A/Texas/04/2009             --MASQGTKRSYEQMETGGERQDATEIRASVGRMIGGIGRFYIQMC 44 A/Mexico/InDRE4487/2009     MSDIEAMASQGTKRSYEQMETGGERQDATEIRASVGRMIGGIGRFYIQMC 50 A/New_York/18/2009          --MASQGTKRSYEQMETGGERQDATEIRASVGRMIGGIGRFYIQMC 44 A/Canada-ON/RV1527/2009     MSDIEAMASQGTKRSYEQMETGGERQDATEIRASVGRMIGGIGRFYIQMC 50 ********************************************

A/California/04/2009        TELKLSDYDGRLIQNSITIERMVLSAFDERRNKYLEEHPSAGKDPKKTGG 94 A/Texas/04/2009             TELKLSDYDGRLIQNSITIERMVLSAFDERRNKYLEEHPSAGKDPKKTGG 94 A/Mexico/InDRE4487/2009     TELKLSDYDGRLIQNSITIERMVLSAFDERRNKYLEEHPSAGKDPKKTGG 100 A/New_York/18/2009          TELKLSDYDGRLIQNSITIERMVLSAFDERRNKYLEEHPSAGKDPKKTGG 94 A/Canada-ON/RV1527/2009     TELKLSDYDGRLIQNSITIERMVLSAFDERRNKYLEEHPSAGKDPKKTGG 100 **************************************************

A/California/04/2009        PIYRRVDGKWMRELILYDKEEIRRVWRQANNGEDATAGLTHIMIWHSNLN 144 A/Texas/04/2009             PIYRRVDGKWMRELILYDKEEIRRVWRQANNGEDATAGLTHIMIWHSNLN 144 A/Mexico/InDRE4487/2009     PIYRRVDGKWMRELILYDKEEIRRVWRQANNGEDATAGLTHIMIWHSNLN 150 A/New_York/18/2009          PIYRRIDGKWMRELILYDKEEIRRVWRQANNGEDATAGLTHIMIWHSNLN 144 A/Canada-ON/RV1527/2009     PIYRRIDGKWMRELILYDKEEIRRVWRQANNGEDATAGLTHIMIWHSNLN 150 *****:********************************************

A/California/04/2009        DATYQRTRALVRTGMDPRMCSLMQGSTLPRRSGAAGAAVKGVGTIAMELI 194 A/Texas/04/2009             DATYQRTRALVRTGMDPRMCSLMQGSTLPRRSGAAGAAVKGVGTIAMELI 194 A/Mexico/InDRE4487/2009     DATYQRTRALVRTGMDPRMCSLMQGSTLPRRSGAAGAAVKGVGTIAMELI 200 A/New_York/18/2009          DATYQRTRALVRTGMDPRMCSLMQGSTLPRRSGAAGAAVKGVGTIAMELI 194 A/Canada-ON/RV1527/2009     DATYQRTRALVRTGMDPRMCSLMQGSTLPRRSGAAGAAVKGVGTIAMELI 200 **************************************************

A/California/04/2009        RMIKRGINDRNFWRGENGRRTRVAYERMCNILKGKFQTAAQRAMMDQVRE 244 A/Texas/04/2009             RMIKRGINDRNFWRGENGRRTRVAYERMCNILKGKFQTAAQRAMMDQVRE 244 A/Mexico/InDRE4487/2009     RMIKRGINDRNFWRGENGRRTRVAYERMCNILKGKFQTAAQRAMMDQVRE 250 A/New_York/18/2009          RMIKRGINDRNFWRGENGRRTRVAYERMCNILKGKFQTAAQRAMMDQVRE 244 A/Canada-ON/RV1527/2009     RMIKRGINDRNFWRGENGRRTRVAYERMCNILKGKFQTAAQRAMMDQVRE 250 **************************************************

A/California/04/2009        SRNPGNAEIEDLIFLARSALILRGSVAHKSCLPACVYGLAVASGHDFERE 294 A/Texas/04/2009             SRNPGNAEIEDLIFLARSALILRGSVAHKSCLPACVYGLAVASGHDFERE 294 A/Mexico/InDRE4487/2009     SRNPGNAEIEDLIFLARSALILRGSVAHKSCLPACVYGLAVASGHDFERE 300 A/New_York/18/2009          SRNPGNAEIEDLIFLARSALILRGSVAHKSCLPACVYGLAVASGHDFERE 294 A/Canada-ON/RV1527/2009     SRNPGNAEIEDLIFLARSALILRGSVAHKSCLPACVYGLAVASGHDFERE 300 **************************************************

A/California/04/2009        GYSLVGIDPFKLLQNSQVVSLMRPNENPAHKSQLVWMACHSAAFEDLRVS 344 A/Texas/04/2009             GYSLVGIDPFKLLQNSQVVSLMRPNENPAHKSQLVWMACHSAAFEDLRVS 344 A/Mexico/InDRE4487/2009     GYSLVGIDPFKLLQNSQVVSLMRPNENPAHKSQLVWMACHSAAFEDLRVS 350 A/New_York/18/2009          GYSLVGIDPFKLLQNSQVVSLMRPNENPAHKSQLVWMACHSAAFEDLRVS 344 A/Canada-ON/RV1527/2009     GYSLVGIDPFKLLQNSQVVSLMRPNENPAHKSQLVWMACHSAAFEDLRVS 350 **************************************************

A/California/04/2009        SFIRGKKVIPRGKLSTRGVQIASNENVETMDSNTLELRSRYWAIRTRSGG 394 A/Texas/04/2009             SFIRGKKVIPRGKLSTRGVQIASNENVETMDSNTLELRSRYWAIRTRSGG 394 A/Mexico/InDRE4487/2009     SFIRGKKVIPRGKLSTRGVQIASNENVETMDSNTLELRSRYWAIRTRSGG 400 A/New_York/18/2009          SFIRGKKVIPRGKLSTRGVQIASNENVETMDSNTLELRSRYWAIRTRSGG 394 A/Canada-ON/RV1527/2009     SFIRGKKVIPRGKLSTRGVQIASNENVETMDSNTLELRSRYWAIRTRSGG 400 **************************************************

A/California/04/2009        NTNQQKASAGQISVQPTFSVQRNLPFERATVMAAFSGNNEGRTSDMRTEV 444 A/Texas/04/2009             NTNQQKASAGQISVQPTFSVQRNLPFERATVMAAFSGNNEGRTSDMRTEV 444 A/Mexico/InDRE4487/2009     NTNQQKASAGQISVQPTFSVQRNLPFERATVMAAFSGNNEGRTSDMRTEV 450 A/New_York/18/2009          NTNQQKASAGQISVQPTFSVQRNLPFERATVMAAFSGNNEGRTSDMRTEV 444 A/Canada-ON/RV1527/2009     NTNQQKASAGQISVQPTFSVQRNLPFERATVMAAFSGNNEGRTSDMRTEV 450 **************************************************

A/California/04/2009        IRMMESAKPEDLSFQGRGVFELSDEKATNPIVPSFDMSNEGSYFFGDNAE 494 A/Texas/04/2009             IRMMESAKPEDLSFQGRGVFELSDEKATNPIVPSFDMSNEGSYFFGDNAE 494 A/Mexico/InDRE4487/2009     IRMMESAKPEDLSFQGRGVFELSDEKATNPIVPSFDMSNEGSYFFGDNAE 500 A/New_York/18/2009          IRMMESAKPEDLSFQGRGVFELSDEKATNPIVPSFDMSNEGSYFFGDNAE 494 A/Canada-ON/RV1527/2009     IRMMESAKPEDLSFQGRGVFELSDEKATNPIVPSFDMSNEGSYFFGDNAE 500 **************************************************

A/California/04/2009        EYDS 498 A/Texas/04/2009             EYDS 498 A/Mexico/InDRE4487/2009     EYDS 504 A/New_York/18/2009          EYDS 498 A/Canada-ON/RV1527/2009     EYDS 504 **** </PRE><SPAN class=3Dpsmall>

multiple sequence alignment of NA
NA CLUSTAL 2.0.10 = multiple sequence alignment of NA

A/California/04/2009        MNPNQKIITIGSVCMTIGMANLILQIGNIISIWISHSIQLGNQNQIETCN 50 A/Texas/04/2009             MNPNQKIITIGSVCMTIGMANLILQIGNIISIWISHSIQLGNQNQIETCN 50 A/Mexico/InDRE4487/2009     MNPNQKIITIGSVCMTIGMANLILQIGNIISIWISHSIQLGNQNQIETCN 50 A/New_York/18/2009          MNPNQKIITIGSVCMTIGMANLILQIGNIISIWISHSIQLGNQNQIETCN 50 A/Canada-ON/RV1527/2009     MNPNQKIITIGSVCMTIGMANLILQIGNIISIWISHSIQLGNQNQIETCN 50 **************************************************

A/California/04/2009        QSVITYENNTWVNQTYVNISNTNFAAGQSVVSVKLAGNSSLCPVSGWAIY 100 A/Texas/04/2009             QSVITYENNTWVNQTYVNISNTNFAAGQSVVSVKLAGNSSLCPVSGWAIY 100 A/Mexico/InDRE4487/2009     QSVITYENNTWVNQTYVNISNTNFAAGQSVVSVKLAGNSSLCPVSGWAIY 100 A/New_York/18/2009          QSVITYENNTWVNQTYVNISNTNFAAGQSVVSVKLAGNSSLCPVSGWAIY 100 A/Canada-ON/RV1527/2009     QSVITYENNTWVNQTYVNISNTNFAAGQSVVSVKLAGNSSLCPVSGWAIY 100 **************************************************

A/California/04/2009        SKDNSVRIGSKGDVFVIREPFISCSPLECRTFFLTQGALLNDKHSNGTIK 150 A/Texas/04/2009             SKDNSVRIGSKGDVFVIREPFISCSPLECRTFFLTQGALLNDKHSNGTIK 150 A/Mexico/InDRE4487/2009     SKDNSVRIGSKGDVFVIREPFISCSPLECRTFFLTQGALLNDKHSNGTIK 150 A/New_York/18/2009          SKDNSIRIGSKGDVFVIREPFISCSPLECRTFFLTQGALLNDKHSNGTIK 150 A/Canada-ON/RV1527/2009     SKDNSIRIGSKGDVFVIREPFISCSPLECRTFFLTQGALLNDKHSNGTIK 150 *****:********************************************

A/California/04/2009        DRSPYRTLMSCPIGEVPSPYNSRFESVAWSASACHDGINWLTIGISGPDN 200 A/Texas/04/2009             DRSPYRTLMSCPIGEVPSPYNSRFESVAWSASACHDGINWLTIGISGPDN 200 A/Mexico/InDRE4487/2009     DRSPYRTLMSCPIGEVPSPYNSRFESVAWSASACHDGINWLTIGISGPDN 200 A/New_York/18/2009          DRSPYRTLMSCPIGEVPSPYNSRFESVAWSASACHDGINWLTIGISGPDN 200 A/Canada-ON/RV1527/2009     DRSPYRTLMSCPIGEVPSPYNSRFESVAWSASACHDGINWLTIGISGPDN 200 **************************************************

A/California/04/2009        GAVAVLKYNGIITDTIKSWRNNILRTQESECACVNGSCFTVMTDGPSNGQ 250 A/Texas/04/2009             GAVAVLKYNGIITDTIKSWRNNILRTQESECACVNGSCFTVMTDGPSNGQ 250 A/Mexico/InDRE4487/2009     GAVAVLKYNGIITDTIKSWRNNILRTQESECACVNGSCFTVMTDGPSNGQ 250 A/New_York/18/2009          GAVAVLKYNGIITDTIKSWRNNILRTQESECACVNGSCFTVMTDGPSDGQ 250 A/Canada-ON/RV1527/2009     GAVAVLKYNGIITDTIKSWRNNILRTQESECACVNGSCFTVMTDGPSDGQ 250 ***********************************************:**

A/California/04/2009        ASYKIFRIEKGKIVKSVEMNAPNYHYEECSCYPDSSEITCVCRDNWHGSN 300 A/Texas/04/2009             ASYKIFRIEKGKIVKSVEMNAPNYHYEECSCYPDSSEITCVCRDNWHGSN 300 A/Mexico/InDRE4487/2009     ASYKIFRIEKGKIVKSVEMNAPNYHYEECSCYPDSSEITCVCRDNWHGSN 300 A/New_York/18/2009          ASYKIFRIEKGKIVKSVEMNAPNYHYEECSCYPDSSEITCVCRDNWHGSN 300 A/Canada-ON/RV1527/2009     ASYKIFRIEKGKIVKSVEMNAPNYHYEECSCYPDSSEITCVCRDNWHGSN 300 **************************************************

A/California/04/2009        RPWVSFNQNLEYQIGYICSGIFGDNPRPNDKTGSCGPVSSNGANGVKGFS 350 A/Texas/04/2009             RPWVSFNQNLEYQIGYICSGIFGDNPRPNDKTGSCGPVSSNGANGVKGFS 350 A/Mexico/InDRE4487/2009     RPWVSFNQNLEYQIGYICSGIFGDNPRPNDKTGSCGPVSSNGANGVKGFS 350 A/New_York/18/2009          RPWVSFNQNLEYQIGYICSGIFGDNPRPNDKTGSCGPVSSNGANGVKGFS 350 A/Canada-ON/RV1527/2009     RPWVSFNQNLEYQIGYICSGIFGDNPRPNDKTGSCGPVSSNGANGVKGFS 350 **************************************************

A/California/04/2009        FKYGNGVWIGRTKSISSRNGFEMIWDPNGWTGTDNNFSIKQDIVGINEWS 400 A/Texas/04/2009             FKYGNGVWIGRTKSISSRNGFEMIWDPNGWTGTDNNFSIKQDIVGINEWS 400 A/Mexico/InDRE4487/2009     FKYGNGVWIGRTKSISSRNGFEMIWDPNGWTGTDNNFSIKQDIVGINEWS 400 A/New_York/18/2009          FKYGNGVWIGRTKSISSRNGFEMIWDPNGWTGTDNNFSIKQDIVGINEWS 400 A/Canada-ON/RV1527/2009     FKYGNGVWIGRTKSISSRNGFEMIWDPNGWTGTDNNFSIKQDIVGINEWS 400 **************************************************

A/California/04/2009        GYSGSFVQHPELTGLDCIRPCFWVELIRGRPKENTIWTSGSSISFCGVNS 450 A/Texas/04/2009             GYSGSFVQHPELTGLDCIRPCFWVELIRGRPKENTIWTSGSSISFCGVNS 450 A/Mexico/InDRE4487/2009     GYSGSFVQHPELTGLDCIRPCFWVELIRGRPKENTIWTSGSSISFCGVNS 450 A/New_York/18/2009          GYSGSFVQHPELTGLDCIRPCFWVELIRGRPKENTIWTSGSSISFCGVNS 450 A/Canada-ON/RV1527/2009     GYSGSFVQHPELTGLDCIRPCFWVELIRGRPKENTIWTSGSSISFCGVNS 450 **************************************************

A/California/04/2009        DTVGWSWPDGAELPFTIDK 469 A/Texas/04/2009             DTVGWSWPDGAELPFTIDK 469 A/Mexico/InDRE4487/2009     DTVGWSWPDGAELPFTIDK 469 A/New_York/18/2009          DTVGWSWPDGAELPFTIDK 469 A/Canada-ON/RV1527/2009     DTVGWSWPDGAELPFTIDK 469 ******************* </PRE><SPAN class=3Dpsmall>

multiple sequence alignment of MP
MP CLUSTAL 2.0.10 = multiple sequence alignment of MP

A/California/04/2009        TEVETYVLSIIPSGPLKAEIAQRLESVFAGKNTDLEALMEWLKTRP 46 A/Texas/04/2009             MSLLTEVETYVLSIIPSGPLKAEIAQRLESVFAGKNTDLEALMEWLKTRP 50 A/New_York/18/2009          MSLLTEVETYVLSIIPSGPLKAEIAQRLESVFAGKNTDLEALMEWLKTRP 50 A/Mexico/InDRE4487/2009     MSLLTEVETYVLSIIPSGPLKAEIAQRLESVFAGKNTDLEALMEWLKTRP 50 A/Canada-ON/RV1527/2009     MSLLTEVETYVLSIIPSGPLKAEIAQRLESVFAGKNTDLEALMEWLKTRP 50 **********************************************

A/California/04/2009        ILSPLTKGILGFVFTLTVPSERGLQRRRFVQNALNGNGDPNNMDRAVKLY 96 A/Texas/04/2009             ILSPLTKGILGFVFTLTVPSERGLQRRRFVQNALNGNGDPNNMDRAVKLY 100 A/New_York/18/2009          ILSPLTKGILGFVFTLTVPSERGLQRRRFVQNALNGNGDPNNMDRAVKLY 100 A/Mexico/InDRE4487/2009     ILSPLTKGILGFVFTLTVPSERGLQRRRFVQNALNGNGDPNNMDRAVKLY 100 A/Canada-ON/RV1527/2009     ILSPLTKGILGFVFTLTVPSERGLQRRRFVQNALNGNGDPNNMDRAVKLY 100 **************************************************

A/California/04/2009        KKLKREITFHGAKEVSLSYSTGALASCMGLIYNRMGTVTTEAAFGLVCAT 146 A/Texas/04/2009             KKLKREITFHGAKEVSLSYSTGALASCMGLIYNRMGTVTTEAAFGLVCAT 150 A/New_York/18/2009          KKLKREITFHGAKEVSLSYSTGALASCMGLIYNRMGTVTTEAAFGLVCAT 150 A/Mexico/InDRE4487/2009     KKLKREITFHGAKEVSLSYSTGALASCMGLIYNRMGTVTTEAAFGLVCAT 150 A/Canada-ON/RV1527/2009     KKLKREITFHGAKEVSLSYSTGALASCMGLIYNRMGTVTTEAAFGLVCAT 150 **************************************************

A/California/04/2009        CEQIADSQHRSHRQMATTTNPLIRHENRMVLASTTAKAMEQMAGSSEQAA 196 A/Texas/04/2009             CEQIADSQHRSHRQMATTTNPLIRHENRMVLASTTAKAMEQMAGSSEQAA 200 A/New_York/18/2009          CEQIADSQHRSHRQMATTTNPLIRHENRMVLASTTAKAMEQMAGSSEQAA 200 A/Mexico/InDRE4487/2009     CEQIADSQHRSHRQMATTTNPLIRHENRMVLASTTAKAMEQMAGSSEQAA 200 A/Canada-ON/RV1527/2009     CEQIADSQHRSHRQMATTTNPLIRHENRMVLASTTAKAMEQMAGSSEQAA 200 **************************************************

A/California/04/2009        EAMEVANQTRQMVHAMRTIGTHPSSSAGLKDDLLENLQAYQKRMGVQMQR 246 A/Texas/04/2009             EAMEVANQTRQMVHAMRTIGTHPSSSAGLKDDLLENLQAYQKRMGVQMQR 250 A/New_York/18/2009          EAMEVANQTRQMVHAMRTIGTHPSSSAGLKDDLLENLQAYQKRMGVQMQR 250 A/Mexico/InDRE4487/2009     EAMEVANQTRQMVHAMRTIGTHPSSSAGLKDDLLENLQAYQKRMGVQMQR 250 A/Canada-ON/RV1527/2009     EAMEVANQTRQMVHAMRTIGTHPSSSTGLKDDLLENLQAYQKRMGVQMQR 250 **************************:***********************

A/California/04/2009        FK 248 A/Texas/04/2009             FK 252 A/New_York/18/2009          FK 252 A/Mexico/InDRE4487/2009     FK 252 A/Canada-ON/RV1527/2009     FK 252 ** </PRE><SPAN class=3Dpsmall>

multiple sequence alignment of NS
NS CLUSTAL 2.0.10 = multiple sequence alignment of NS

A/California/04/2009        MDSNTMSSFQVDCFLWHIRKRFADNGLGDAPFLDRLRRDQKSLKGRGNTL 50 A/Texas/04/2009             MDSNTMSSFQVDCFLWHIRKRFADNGLGDAPFLDRLRRDQKSLKGRGNTL 50 A/Mexico/InDRE4487/2009     MDSNTMSSFQVDCFLWHIRKRFADNGLGDAPFLDRLRRDQKSLKGRGNTL 50 A/New_York/18/2009          MDSNTMSSFQVDCFLWHIRKRFADNGLGDAPFLDRLRRDQKSLKGRGNTL 50 A/Canada-ON/RV1527/2009     MDSNTMSSFQVDCFLWHIRKRFADNGLGDAPFLDRLRRDQKSLKGRGNTL 50 **************************************************

A/California/04/2009        GLDIETATLVGKQIVEWILKEESSETLRMTIASVPTSRYLSDMTLEEMSR 100 A/Texas/04/2009             GLDIETATLVGKQIVEWILKEESSETLRMTIASVPTSRYLSDMTLEEMSR 100 A/Mexico/InDRE4487/2009     GLDIETATLVGKQIVEWILKEESSETLRMTIASVPTSRYLSDMTLEEMSR 100 A/New_York/18/2009          GLDIETATLVGKQIVEWILKEESSETLRMTIASVPTSRYLSDMTLEEMSR 100 A/Canada-ON/RV1527/2009     GLDIETATLVGKQIVEWILKEESSETLRMTIASVPTSRYLSDMTLEEMSR 100 **************************************************

A/California/04/2009        DWFMLMPRQKIIGPLCVRLDQAIMEKNIVLKANFSVIFNRLETLILLRAF 150 A/Texas/04/2009             DWFMLMPRQKIIGPLCVRLDQAIMEKNIVLKANFSVIFNRLETLILLRAF 150 A/Mexico/InDRE4487/2009     DWFMLMPRQKIIGPLCVRLDQAIMEKNIVLKANFSVIFNRLETLILLRAF 150 A/New_York/18/2009          DWFMLMPRQKIIGPLCVRLDQAVMEKNIVLKANFSVIFNRLETLILLRAF 150 A/Canada-ON/RV1527/2009     DWFMLMPRQKIIGPLCVRLDQAVMEKNIVLKANFSVIFNRLETLILLRAF 150 **********************:***************************

A/California/04/2009        TEEGAIVGEISPLPSLPGHTYEDVKNAVGVLIGGLEWNGNTVRVSENIQR 200 A/Texas/04/2009             TEEGAIVGEISPLPSLPGHTYEDVKNAVGVLIGGLEWNGNTVRVSENIQR 200 A/Mexico/InDRE4487/2009     TEEGAIVGEISPLPSLPGHTYEDVKNAVGVLIGGLEWNGNTVRVSENIQR 200 A/New_York/18/2009          TEEGAIVGEISPLPSLPGHTYEDVKNAVGVLIGGLEWNGNTVRVSENIQR 200 A/Canada-ON/RV1527/2009     TEEGAIVGEISPLPSLPGHTYEDVKNAVGVLIGGLEWNGNTVRVSENIQR 200 **************************************************

A/California/04/2009        FAWRNCDENGRPSLPPEQK 219 A/Texas/04/2009             FAWRNCDENGRPSLPPEQK 219 A/Mexico/InDRE4487/2009     FAWRNCDENGRPSLPPEQK 219 A/New_York/18/2009          FAWRNCDENGRPSLPPEQK 219 A/Canada-ON/RV1527/2009     FAWRNCDENGRPSLPPEQK 219 ******************* </PRE><SPAN class=3Dpsmall>